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APPLICATION OF MICROTECHNOLOGY IN BIOTECHNOLOGY. MICROARRAY ANALYTICAL SYSTEMS – AN OVERVIEW

G. De Bellis1, G. Caramenti1, M. Ilie2,3,* , E. Cianci2, V. Foglietti2

Affiliation

  1. Istituto di Tecnologie Biomediche (ITB)-CNR, Via Fratelli Cervi 93, 20090 Segrate, Italy
  2. Istituto di Elettronica dello Stato Solido (IESS)-CNR, Via Cineto Romano 42, 00156 Roma, Italy
  3. University ‘Politehnica’ of Bucharest, Bd. Iuliu Maniu 1-6, Bucharest, Romania

Abstract

The evolution of modern biology, that recently entered the genome era, requires faster and cheaper analytical systems. Techniques as microlithography, micromachining, laser induced fluorescence detection, microfluidics contributed to the development of the DNA microarray technology, having as result complex analytical systems able to perform hybridization of nucleic acids in an array format on small bidimensional surfaces, and also to process huge quantities of information. The most significant achievements worldwide obtained are presented illustrating the state of the art in this domain. In order to automate the fluidic process involved in the DNA hybridization three micromachining techniques, slightly differing from each other have been approached by the authors team. Reservoirs with volumes ranging from 1nl to 2 ml in different materials have been obtained by means of reactive ion etching of polyimide, anisotropic etching of silicon respectively an optimised wet etching of borosilicate glass as well as a low temperature bonding of borosilicate glass on silicon nitride. One obtained device has been tested in a Laser Induced Fluorescence detection set-up..

Keywords

Analytical system, Micromachined reservoirs, Laser induced fluorescence.

Submitted at: Oct. 28, 2002
Accepted at: March 12, 2003

Citation

G. De Bellis, G. Caramenti, M. Ilie, E. Cianci, V. Foglietti, APPLICATION OF MICROTECHNOLOGY IN BIOTECHNOLOGY. MICROARRAY ANALYTICAL SYSTEMS – AN OVERVIEW, Journal of Optoelectronics and Advanced Materials Vol. 5, Iss. 1, pp. 89-96 (2003)