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Auto-fluorescence spectroscopy of colorectal carcinoma: ex vivo study

L. HORAK1,* , J. ZAVADIL2, V. DUCHAC1, S. JAVORSKY2, F. KOSTKA2, A. SVEC3, D. LEZAL4

Affiliation

  1. Surgical Clinic of the IIIrd Medical Faculty, Charles University, Prague, Srobarova 55, 100 00 Praha 10, Czech Republic
  2. Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, 182 51 Praha 8, Czech Republic
  3. Institute of Pathology, of the IIIrd Medical Faculty of the Charles University Prague, Šrobárova 55, 100 00 Praha 10, Czech Republic
  4. Institute of Inorganic Chemistry, Academy of Sciences of the Czech Republic, 250 68 Rez u Prahy, Czech Republic

Abstract

Ex-vivo investigation of colorectal tissue by autofluorescence spectroscopy is reported. Wet microscopic mounts of healthy colon mucosa were examined and compared with those prepared from colon mucosa affected by adenocarcinoma. The diagnosis of adenocarcinoma was verified by using clinical and histology means. Fluorescence spectra of tissue samples, excited by blue emitting semiconductor diode have been studied by using portable spectrometer with fixed grating and fibre optic system compatible with endoscope. The study demonstrates differences in both the spectral shape and in the signal intensity in photoluminescence spectra emitted from tissue affected by adenocarcinoma as compared to that of healthy colon mucosa. The overall decrease and the shift of autofluorescence intensity to longer wavelengths have been observed for malignant tissue samples..

Keywords

Autofluorescence spectroscopy, Carcinoma.

Submitted at: Jan. 24, 2006
Accepted at: Jan. 26, 2006

Citation

L. HORAK, J. ZAVADIL, V. DUCHAC, S. JAVORSKY, F. KOSTKA, A. SVEC, D. LEZAL, Auto-fluorescence spectroscopy of colorectal carcinoma: ex vivo study, Journal of Optoelectronics and Advanced Materials Vol. 8, Iss. 1, pp. 396-399 (2006)