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DYNAMIC LIGHT SCATTERING: A USEFUL OPTICAL METHOD TO PROBE COMMON-ION EFFECTS IN PROTEIN-SALT AQUEOUS SOLUTIONS

D. Buzatu1,* , O. Annunziata2, E. Petrescu1, C. Popa1, F. D. Buzatu3

Affiliation

  1. Physics Department, “Politehnica” University, Bucharest, 77206, Romania
  2. Texas Christian University, Chemistry Department, Fort Worth, Texas 76129, USA
  3. Department of Theoretical Physics, National Institute for Physics and Nuclear Engineering, Bucharest-Magurele, 76900, Romania

Abstract

We report measurements of protein diffusion coefficients for lysozyme aqueous solutions using dynamic light scattering (DLS). DLS measurements were performed on the buffer-free lysozyme-NaCl-water and lysozyme-Na2SO4-water ternary systems at pH 4.5 and 25 ºC. The dependence of lysozyme diffusion coefficients as a function of salt concentration is analyzed. We find that the behavior of the protein diffusion coefficient in the presence of Na2SO4 is significantly different from that in the presence of NaCl. Our DLS measurements show that the common-ion effect plays an important role in the case of lysozyme-NaCl solutions but not in the case of lysozyme-Na2SO4 solutions. Therefore DLS is a useful optical method that can be used to probe the presence of common-ion effects in protein-salt aqueous solutions..

Keywords

Dynamic light scattering, Lysozyme-Na2SO4-H2O, Diffusion coefficient.

Submitted at: Sept. 29, 2005
Accepted at: Nov. 24, 2005

Citation

D. Buzatu, O. Annunziata, E. Petrescu, C. Popa, F. D. Buzatu, DYNAMIC LIGHT SCATTERING: A USEFUL OPTICAL METHOD TO PROBE COMMON-ION EFFECTS IN PROTEIN-SALT AQUEOUS SOLUTIONS, Journal of Optoelectronics and Advanced Materials Vol. 7, Iss. 6, pp. 3161-3168 (2005)