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Apoptotic effect of TiO2 in HepG2 cellular model

MUHAMMAD FAKHAR-E-ALAM1, NAJEEB ABBAS1, MUHAMMAD IMRAN2, M. ATIF3,4,*

Affiliation

  1. Department of Physics, Faculty of Science and Technology, GC University, Faisalabad, Pakistan
  2. School of chemical and Materials Engineering, NUST, Islamabad Pakistan
  3. Department of Physics and Astronomy, King Saud University, Riyadh, Saudi Arabia
  4. National Institute of Laser and Optronics, Nilore, Islamabad, Pakistan

Abstract

Liver cancer being the fifth most common lethal cancer in the world requires prompt emergent, convincing, effective treatment, which can be acquired by the PDT. Current study demonstrates the cytotoxic response of TiO2 NPs in hepatocellular (HepG2) model. However, underlying mechanism of apoptosis in HepG2 cells due to TiO2 NPs exposure is largely lacking. We explored the possible mechanism of apoptotic effect induced by titanium dioxide nanoparticles in human hepatocellular model. Due to high surface to volume ratio and extremely toxic nature, said nanomaterial can be used as tumoricidal drugs which can be act/proved as milestone for cancer treatment. TiO2 NPs were prepared via Sol-Gel technique by using Titanium Isopropoxide as a main precursor. Crystal structures of the particles were examined XRD and the surface morphology of synthesized nanoparticles investigated by SEM. In said research work, focus of our study was to explore the actual cytotoxicity and photo-toxicity of TiO2 NPs individually having broad range concentration when expose to HepG2 cellular model for 48 hours as time of spam. After successful investigation of toxicity of said nanoparticles in said cellular model, the author is confident to demonstrate the actual reason of cell killing mechanism which will be big turnip of scientists, might be helpful especially for real treatment of malignant/pre-malignant patients. Additionally, a comparison of cytotoxicity between ZnO and TiO2 nanoparticles is also explored in this article..

Keywords

Titanium dioxide nanoparticles (TiO2 NPs), Hepatocellular carcinoma (HePG2 cell line), X-ray diffraction (XRD), scanning electron microscopy (SEM).

Submitted at: Sept. 30, 2014
Accepted at: Nov. 13, 2014

Citation

MUHAMMAD FAKHAR-E-ALAM, NAJEEB ABBAS, MUHAMMAD IMRAN, M. ATIF, Apoptotic effect of TiO2 in HepG2 cellular model, Journal of Optoelectronics and Advanced Materials Vol. 16, Iss. 11-12, pp. 1481-1486 (2014)