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Cryoprotector effect on main properties of lipid nanoparticles loaded with bio-active compounds

I. LACATUSU1, M. N. BADEA1,* , A. M. MANEA1, A. MEGHEA1

Affiliation

  1. Faculty of Applied Chemistry and Materials Science, University POLITEHNICA of Bucharest, Polizu Street No 1, 011061, Bucharest, Romania

Abstract

The paper is devoted to the investigation of influence of mannitol and trehalose used as cryoprotectors on the main properties of lipid nanoparticles loaded with two kinds of active compounds: an UV molecular absorber (octocrylen) and a natural antioxidant (carotene). The lipid nanoparticles loaded with the selected active compounds were characterized for their physical stability (electrokinetic potential measurements) together with evaluation of size distribution and polydispersity index, by using the dynamic light scattering technique. The crystallization occurred in the inner core of lipid nanoparticles was also investigated by differential scanning calorimetry. Appropriate in vitro determination methods have been used in order to evaluate the antioxidant and anti-UV properties of lipid nanoparticles. All experimental results have been shown that a good photoprotection effect and improved antioxidant activities could be obtained by using such cryoprotector agents. By determining the ability of carotene – lipid nanoparticles to scavenge free radicals, it has been shown that the presence of trehalose produced a significant effect on the antioxidant behaviour of nanoparticles. Regarding the anti-UV properties, a concentration over than 5% mannitol has lead to a gradually decreasing of SPF values, while 2.5% trehalose have demonstrated an increasing of SPF index..

Keywords

Lipid nanoparticles, Cryoprotectors, Octocrylen, Carotene, Antioxidant activity, Photoprotective properties.

Submitted at: Nov. 30, 2011
Accepted at: April 11, 2012

Citation

I. LACATUSU, M. N. BADEA, A. M. MANEA, A. MEGHEA, Cryoprotector effect on main properties of lipid nanoparticles loaded with bio-active compounds, Journal of Optoelectronics and Advanced Materials Vol. 14, Iss. 3-4, pp. 336-343 (2012)