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DNA structural information from Giardia intestinalis tpi gene assemblages using the wavelet spectrogram analysis

I. M. NEAGOE1,2, S. MICLOS3,* , D. POPESCU4,5, D. SAVASTRU3, D. STERIU1,2, S. DONTU3, V. I. R. NICULESCU6, M. TAUTAN3

Affiliation

  1. Department of Parasitology, "Carol Davila" University of Medicine and Pharmacy, D.Gerota 19-21, Bucharest, Romania
  2. National Institute of Research and Development for Microbiology and Immunology "Cantacuzino", Spl. Independentei 103, Bucharest, Romania
  3. National Institute of R&D for Optoelectronics - INOE 2000, 409 Atomistilor St., Magurele, Ilfov, RO-077125, Romania
  4. Department of Mathematical Modelling in Life Sciences, Institute of Mathematical Statistics and Applied Mathematics of Romanian Academy, Calea 13 Septembrie 13, Bucharest, Romania
  5. Department of Physiology and Biophysics, Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, Bucharest, Romania
  6. National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor St., P.O.Box MG-36, Bucharest-Magurele, RO-077125, Romania

Abstract

Molecular investigations of Giardia intestinalis tpi polymorphic gene have facilitated approaching of some mathematical methods to describe the genetic information. In this study, we applied wavelet spectrogram analysis for estimating the degree of similarity and difference between different assemblages and intra-assemblages of infectious parasite Giardia intestinalis. Five different DNA sequences were investigated, of which two sample sequences were isolated, sequenced and analyzed in our laboratory and subsequently deposited in GenBank. Using additional a quantitative comparison index and Multidimensional Scaling as visualization method we revealed some characteristics of DNA information embedded in the analyzed tpi sequences..

Keywords

Wavelet spectrogram analysis, Multidimensional Scaling, DNA sequence, Giardia tpi gene; genetic assemblages.

Submitted at: Dec. 5, 2013
Accepted at: March 13, 2014

Citation

I. M. NEAGOE, S. MICLOS, D. POPESCU, D. SAVASTRU, D. STERIU, S. DONTU, V. I. R. NICULESCU, M. TAUTAN, DNA structural information from Giardia intestinalis tpi gene assemblages using the wavelet spectrogram analysis, Journal of Optoelectronics and Advanced Materials Vol. 16, Iss. 3-4, pp. 408-413 (2014)