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Low resolution structural studies of apoferritin via SANS and SAXS: the effect of concentration

T. N. MURUGOVA1,2, A. V. VLASOV2, O. I. IVANKOV1,3, A. V. ROGACHEV1,2, YU. L. RYZHYKAU2, D. V. SOLOVIOV1,3, A. ZHIGUNOV4, E. V. ZINOVEV2, YU. S. KOVALEV1,2, A. ROUND5, V. I. GORDELIY1,2,6,7, A. I. KUKLIN1,2,*

Affiliation

  1. Joint Institute for Nuclear Research, Dubna, Russia
  2. Moscow Institute of Physics and Technology, Dolgoprudniy, Russia
  3. Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
  4. Institute of Macromolecular Chemistry CAS, Prague, Czech Republic
  5. EMBL, 6 Jules Horowitz, F-38042 Grenoble, France
  6. Institute of Complex Systems (ICS), ICS-5: Molecular Biophysics, Research Centre Juelich, 52425, Juelich, Germany
  7. Institute of Structural Biology J.P.Ebel, Grenoble, France

Abstract

The results of small angle scattering investigation of protein apoferritin are presented. In particular, the results of approximation to zero value of concentration are calculated. The procedure of approximating at zero concentration of apoferritin in this work is considered. The sizes and shapes, including those determined by indirect Fourier transform method, obtained on different instruments are compared. The pair-distance distribution function for both small angle neutron (SANS) and X-ray scattering (SAXS) is computed. It is shown that SANS and SAXS methods give similar form factor (spherical shell with holes) of apoferritin. At the same time, fits of experimental data for SAXS and SANS curves give different sizes and volumes of the molecule. The reasons for these discrepancies are discussed..

Keywords

Small-angle X-ray and neutron scattering, Apoferritin, Concentration effect, Instrument comparison.

Submitted at: Aug. 17, 2015
Accepted at: Sept. 9, 2015

Citation

T. N. MURUGOVA, A. V. VLASOV, O. I. IVANKOV, A. V. ROGACHEV, YU. L. RYZHYKAU, D. V. SOLOVIOV, A. ZHIGUNOV, E. V. ZINOVEV, YU. S. KOVALEV, A. ROUND, V. I. GORDELIY, A. I. KUKLIN, Low resolution structural studies of apoferritin via SANS and SAXS: the effect of concentration, Journal of Optoelectronics and Advanced Materials Vol. 17, Iss. 9-10, pp. 1397-1402 (2015)