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Oxidative stress studies on plant DNA exposed to ozone

M. E. BARBINTA PATRASCU1, N. BADEA2,* , A. MEGHEA2

Affiliation

  1. Faculty of Physics, University of Bucharest, Atomistilor Street, Magurele, CP MG-11, RO- 077125, Romania
  2. Faculty of Applied Chemistry and Materials Science, University POLITEHNICA of Bucharest, Polizu Street No 1, 011061, Bucharest, Romania

Abstract

This work is centered on the study of the behaviour of the plant DNA under conditions of oxidative degradation by ozone. The total DNA of high purity was extracted from fresh sunflower leaves. Using a system of free radicals generated in vitro, the action of reactive oxygen species on DNA was tested by combining spectrophotometrical technique with chemiluminescent assay and Polymerase Chain Reaction (PCR). The ozone and the reactive oxygen species have a high potential to interact with DNA molecules resulting in structural modifications and possible denaturations seriously affecting the cell function..

Keywords

Oxidative stress, Deoxyribonucleic acid, Ozone, Polymerase Chain Reaction.

Submitted at: Jan. 18, 2013
Accepted at: June 12, 2013

Citation

M. E. BARBINTA PATRASCU, N. BADEA, A. MEGHEA, Oxidative stress studies on plant DNA exposed to ozone, Journal of Optoelectronics and Advanced Materials Vol. 15, Iss. 5-6, pp. 589-594 (2013)