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Physico-chemical and antibacterial studies on silver doped nano-hydroxyapatite

C.S. CIOBANU1, E. ANDRONESCU2,3, A.M. PRODAN2,4,5, L. PALL2,6, A. COSTESCU7,8,9, P. LE COUSTUMER8, F. HUNEAU9,10, L. MARUTESCU11, N.I. ENE11, R. TRUSCA9, E. S. BARNA7, S. L. ICONARU1,7,*

Affiliation

  1. National Institute of Materials Physics, 105 bis Atomistilor, P.O. Box MG 07, 077125, Bucuresti-Magurele, Romania
  2. University Politechnica of Bucharest, Faculty of Applied Chemistry and Materials Science,1-7 Polizu Street, P.O. Box 12-134, 011061 Bucharest, Romania
  3. AOSR, Splaiul Independentei nr. 54, sector 5, 050094 Bucuresti, Romania
  4. Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari, sector 5, Bucharest, Romania
  5. Emergency Hospital Floreasca, 8 Calea Floresca, sector 1, Bucharest, Romania fICMCB-CNRS, UPR9048, 87 Avenue du Docteur Schweitzer 33608, Pessac Cedex France
  6. ICMCB-CNRS, UPR9048, 87 Avenue du Docteur Schweitzer 33608, Pessac Cedex France
  7. University of Bucharest, Faculty of Physics, 405 Atomistilor, CP MG – 1, 077125, Bucuresti-Magurele, Romania
  8. Universite Bordeaux, EA 4592 Géoressources & Environnement, EGID, 1 allée F. Daguin 18, 33607 Pessac Cedex France
  9. Universite de Corse Pascal Paoli, Faculté des Sciences et Techniques, Laboratoire d'Hydrogéologie, F 20250 Corte, France
  10. CNRS UMR 6134 SPE, , F 20250 Corte, FrancekFaculty of Biology, Microbiology Department, University of Bucharest, Ale. Portocalelor 1-3, 60101, Bucharest, Romania
  11. SC Metav-CD SA, 31 C.A. Rosetti , Bucharest, Romania

Abstract

In this paper we show that preparation of Ag doped hydroxyapatite by an adapted co-precipitation method at 100°C has several advantages over other techniques. Specifically, it can generate highly crystalline nanopowder Ag:HAp which could be used for implantable medical devices. The XRD of HAp (xAg = 0) and Ag:HAp (xAg = 0.05, and xAg = 0.4) also demonstrates that powders obtained by co-precipitation at 1000C exhibit the apatite characteristics with good crystal structure and no new phase or impurity is found. The SEM results suggested that Ag+ doping had little influence on the morphology and dimension of the samples. It can be seen that all the samples consist of elipsoidal particles. The antibactericidal activity of Ag:HAp-NPs with xAg = 0, xAg = 0.05, and xAg = 0.4 on Bacilus and E.coli ESBL 1576 were presented. The Ag:HAp-NPs with xAg = 0.05, and xAg = 0.4 inhibited the biofilm development both by the gram-positive (Staphylococcus aureus 0364) and the gram-negative (Providencia stuartii 1116) strains. On the other hand, our studies have shown that Ag:HAp with xAg = 0 had no antibacterial activity against gram-positive and gram-negative bacteria..

Keywords

Silver, Hydroxyapatite, Antibacterial activity.

Submitted at: March 25, 2013
Accepted at: July 11, 2013

Citation

C.S. CIOBANU, E. ANDRONESCU, A.M. PRODAN, L. PALL, A. COSTESCU, P. LE COUSTUMER, F. HUNEAU, L. MARUTESCU, N.I. ENE, R. TRUSCA, E. S. BARNA, S. L. ICONARU, Physico-chemical and antibacterial studies on silver doped nano-hydroxyapatite, Journal of Optoelectronics and Advanced Materials Vol. 15, Iss. 7-8, pp. 918-922 (2013)