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The efficacy of photodynamic inactivation of the microorganisms using laser sources and methylene blue as sensitizer

M. A. CALIN1, R. M. ION2,3,*

Affiliation

  1. National Institute of Research and Development for Optoelectronics INOE 2000, Magurele, Romania
  2. National Institute of Research and Development for Chemistry and Petrochemistry – ICECHIM, Bucharest, Romania
  3. Valahia University, Targoviste, Romania

Abstract

The large spread of pathogen microorganisms in nature and their increasing resistence to drugs, lead to alternative treatment methods, more efficient and without microbial resistance. One of these methods is the photodynamic inactivation of microorganisms. In the present paper, we are discussing the efficacity of the laser system SCL (INOE 2000, P = 15 mW, λ = 635 nm) on yeast cells (Saccharomyces cerevisiae - as model system) treated with methylene blue as sensitizer. Diffuse reflectance spectroscopy and Kramers – Kronig analysis have been used for in vitro determination of the refractive index changes of yeast cells induced by laser irradiation..

Keywords

Saccharomyces cerevisiae, Photodynamic effect, Photosensitizer, Laser source, Diffuse reflectance spectrometry, Refractive index, Microscopy.

Submitted at: Dec. 15, 2009
Accepted at: Jan. 20, 2010

Citation

M. A. CALIN, R. M. ION, The efficacy of photodynamic inactivation of the microorganisms using laser sources and methylene blue as sensitizer, Journal of Optoelectronics and Advanced Materials Vol. 12, Iss. 1, pp. 141-145 (2010)